p2x 7 control antigen  (Alomone Labs)

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Immunohistochemical detection of P2X receptor isoforms. The figure shows subtype specific staining for P2X1, P2X4, P2X5 and P2X7 receptors, reduction in staining with the appropriate blocking peptide controls and the lack of specific staining for P2X2 and P2X3 receptors. P2X1,4 and P2X5 receptor immunoreactivity was restricted to the smooth muscle layer. P2X7 receptor immunoreactivity was punctate (indicated by arrow) and restricted to the outer adventitial layer.

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques: Immunohistochemical staining, Staining, Blocking Assay

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Run-down of P2X receptor currents in the whole cell recording mode. (A) α,β-meATP (10 μM) evokes transient inward currents which desensitize rapidly during agonist application (200 ms, application indicated by bar). The peak amplitude of currents ‘ran-down' during repeated applications of α,β-meATP at 5 min intervals. (B) The timecourse of run-down of P2X currents in the whole cell recording mode. Recovery from desensitization is maximal with a 3–5 min interval between applications.

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques:

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Reproducible P2X receptor currents in response to α,β-meATP (10 μM, 200 ms application indicated by bar) were recorded in the amphotericin permeabilized patch recording configuration when the interval between applications was 5 mins (A). (B) Timecourse of recovery from desensitization of P2X receptor currents, expressed as per cent of the initial response to α,β-meATP (10 μM, n=4–6 for each point).

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques:

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Current-voltage relationship of P2X receptor currents in rat mesenteric artery smooth muscle cells. (A) Currents evoked by α,β-meATP (10 μM, application period indicated by bar) showed inward rectification (B). (C) Mono-exponential fit of the decay of a P2X receptor response to the continued application of α,β-meATP (10 μM).

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques:

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Effects of nucleotides at rat mesenteric artery P2X receptors. Sample traces recorded using the permeabilized patch configuration of responses to α,β-meATP (A), l-β,γ-meATP (B) and CTP (C). Commercially available ADP (100 μM) appeared an effective agonist but when purified activity was reduced by >95% (responses to 10 μM α,β-meATP indicated by • in B–D). Agonist application indicated by bar.

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques: Purification, Activity Assay

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Concentration response relationships for a range of nucleotides at rat mesenteric artery P2X receptors showing ATP, 2meSATP, α,β-meATP, l-β,γ-meATP, UTP, GTP, TTP, and ITP. All data are expressed as per cent of response to 10 μM α,β-meATP, n=4–11 for each point.

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques: Concentration Assay

Journal:

Article Title: Lack of run-down of smooth muscle P2X receptor currents recorded with the amphotericin permeabilized patch technique, physiological and pharmacological characterization of the properties of mesenteric artery P2X receptor ion channels

doi: 10.1038/sj.bjp.0703744

Figure Lengend Snippet: Antagonism of P2X receptor-mediated currents in rat mesenteric artery smooth muscle cells. (A) The response to α,β-meATP (3 μM) is abolished by 30 μM suramin, note the potentiation of the response on washout. (B) iso-PPADS (1 μM) abolished the response to α,β-meATP (3 μM), this effect was partially reversed after 5 mins washout. (C) Summary of the concentration dependence of inhibition of α,β-meATP (3 μM) evoked responses by the antagonists suramin and iso-PPADS. (n=4–6 for each point).

Article Snippet: Transverse sections (12 μm) of mesenteric arteries were processed for immunochemistry as described previously ( Lewis et al ., 2000 ) using the following P2X receptor subtype selective antibodies; anti-P2X 1 , anti-P2X 2 , anti-P2X 4 and anti-P2X 7 ±control antigen blocking peptide (Alomone Lab. Israel); anti-P2X 3 (gift from Prof Elde and Dr Vulchanova, University of Minnesota, U.S.A.), anti-P2X 5 and anti-P2X 6 ±control antigen blocking peptide (gift from Roche Bioscience, Palo Alto, U.S.A.).

Techniques: Concentration Assay, Inhibition